Abstract
ObjectivesEmerging data suggest that several metabolic factors, released mainly by white adipose tissue (WAT) and joint tissues, and collectively named adipokines, might have a role in the pathophysiology of OA. Recently, novel adipokines such as SERPINE2, WISP2, GPNMB and ITIH5 have been identified in WAT. The main goal of this study was to analyse the expression of these novel adipokines in synovium, infrapatellar fat pad and chondrocytes and to compare the expression of these molecules in healthy and OA tissues.MethodsSynovial tissues, infrapatellar fat pad and chondrocytes were obtained from 36 OA patients (age 52–85; mean BMI 28.9) who underwent total knee replacement surgery. Healthy synovial tissues and infrapatellar fat pad were obtained from 15 traumatic knee patients (age 23–53; mean BMI 23.5). mRNA and protein expression were determined by qRT-PCR and western blot analysis respectively.ResultsAll the novel adipokines, matter of our study, are expressed in OA synovium, infrapatellar fat pad and chondrocytes. Moreover, we detected a differential expression of SERPINE2 and ITIH5 in OA synovial tissues as compared to healthy samples. Finally, we also observed an increased expression of WISP2 in OA infrapatellar fat pad in comparison to healthy controls.ConclusionsIn this study we demonstrated for the first time the expression of four novel adipokines in different joint tissues and how these molecules are differentially expressed in healthy and OA joint tissues.
Highlights
Osteoarthritis (OA) is one of the most common form of arthritis and a major cause of pain and disability in adult population
In this study we demonstrated for the first time the expression of four novel adipokines in different joint tissues and how these molecules are differentially expressed in healthy and OA joint tissues
OA was first considered a disorder of the articular cartilage, nowadays it is generally recognized that OA affects all joint tissues, including synovium, ligaments, tendons, muscle and subchondral bone [1]
Summary
Osteoarthritis (OA) is one of the most common form of arthritis and a major cause of pain and disability in adult population. Several risk factors contribute to osteoarthritis development, including sex, age, mechanical factors or obesity, among others. Due to the increased fat mass, obesity enhances mechanical stress in weight bearing joints, and contributes to joint tissues degeneration by producing and releasing a plethora of factors called adipokines [2]. Adipose tissue is currently considered a very active endocrine organ able to secrete many factors which could participate in the pathophysiology of OA [2]. Most of these molecules are produced and secreted by joint cell populations such as chondrocytes and/or synovial fibroblasts [3,4]
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