Identification of novel 1, 3-oxazole and imidazole-5-one that inhibits bacterial biofilm formation of Acinetobacter baumannii

Abstract

Bacterial biofilms are chargeable for many chronic infections, including caries, osteomyelitis, and CF. owing to an incredible antimicrobial resistance of biofilms, bacterial species sustain from immune defense and antiseptic agents, seeking and development of novel approaches to hinder the biofilm formation seems pivotal. In this study, a novel 1,3-Oxazole-5-one mixes 2(a-c) have been set up by cyclization of compound (1) with aromatic aldehydes subsidiaries. The beginning compound (1) was promptly acquired by the response of 4-fluoro benzoyl chloride with glycine. Every new compound was portrayed by Proton atomic attractive reverberation (1H NMR), Fourier changes infrared spectroscopy (FTIR) and Ultraviolet (UV) spectroscopy, named antibiofilm compound 6, was found to effect on bacterial biofilm formation in Acinetobacter baumannii. Results of statistical analysis revealed that there were significant differences between the rate of biofilm formation of test isolates and control isolate at a significant level of P > 0.01. Gene expression for biofilm encoding genes and mqsR gene for selected treated and non-treated isolates were evaluated, The results showed a difference in the gene expression between biofilm genes when the ompA has shown a high genetic expression in contrast to the bap and mqsR genes, which gave a little gentic expression after treated these selected isolates with chemical compound compared with control levels. These data suggest antibiofilm compound 6 is a novel efficient chemical scaffold.