Identification of non-coding RNAs in the hyper thermophilic bacterium Thermotoga maritima MSB8 through comparative genomics and in-silico analyses

doi:10.35495/ajab.2021.05.224

Abstract

Highlights

A hyperthermophilic rod like bacterium Thermotoga maritima was isolated (Singh et al, 2015) which can grow between the temperature range of 60-90°C with optimum growth at 80°C (Huber et al, 1986)
This study was based on a comparativegenomics approach for the genome-wide detection of small noncoding RNAs (ncRNAs) in T. maritima and different bacterial genomes
The novel ncRNA candidates were detected in T. maritima and 20 bacterial genomes after subjecting them to a series of bioinformatics tools and following a comparative genomics-based approach

Summary

Introduction

Reactive environment of the hydrothermal vents supports different ecosystems and provides optimum conditions for the stable synthesis of prebiotics (Martin et al, 2008). The hyperthermophilic nature of T. maritima makes it an attention grabber for the production of various industrially important biocatalysts including esterases, lipases, and amylases, because of their extremely high thermal stability and efficiency over other enzymes for hightemperature industrial processes (Levisson et al, 2007; Mehmood et al, 2014) This bacterium has been the focus of many evolutionary biologists because it harbors the characteristics of early microorganisms on earth and can produce a high amount of biohydrogen by fermentation (Singh et al, 2015), along with acetic acid and carbon dioxide. Like different other organisms which produce heat shock proteins (Lindquist and Craig, 1988), MSB8 tends to incorporate more “charged” amino acids on the surfaces of their proteins, to form an impenetrable hydrophobic core (Kumar and Nussinov, 2001) This bacterium has a sheet-like structure or toga present on its poles, made up of porin (OmpB), and an alphahelical protein (OmpA1) (Ranjit and Noll, 2016) forming a trimer of proteins, enclosing the whole bacterium. Different structural and genetic properties have been associated with the thermostability of T. maritima, but due to the huge and complex structural and genomic data, the real picture is still unclear

Methods

Results

Discussion

Conclusion