Abstract
We have studied the presence of neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS) in parenchymal and pial bovine cerebral vessels by using western blot analysis. The different vessel structures were analysed by microscopic observation and their biochemical features determined by using γ-glutamiltranspeptidase (γ-GTP) as an endothelial marker and α-smooth muscle actin (α-SMA) as a smooth muscle cell marker. nNOS could not be found in parenchymal vessels, being present only in pial vessels; eNOS was present in all vessel-fractions studied, albeit a different distribution was found. Thus, the eNOS is more abundant in the parenchymal vessels which are associated with smooth muscle cells whereas both pial vessels and brain microvessels, almost devoid of actin, show extremely low levels. Two main conclusions can be obtained: first, nNOS is exclusively restricted to pial vessels and, second, eNOS is present in endothelial cells which are in association with smooth muscle cells.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
