IDENTIFICATION OF NEW SNPS IN GENES/LOCI OF RESISTANCE TO FUSARIOSIS AND CHITINASE

Abstract

The aim of this work is to test specific primers FusA1F/R, 2Ch300F/R, and 7Ch310F/R to study SE2 gene responsible for expression of acid chitinase in stressful situations, and loci that ensure resistance of sugar beetroot breeding material to Fusarium rot. Material for the study was sugar beetroot plants of domestic and foreign selection. To confirm the connection of SE2 gene (localized on chromosome 3)which controlsstable level of acid chitinase with resistance of sugar beetroot to root rot, ten samples of sugar beetroot were typed using molecular genetic marker FusA1. Two 600 b.p. and 400 b.p. DNA fragments in all studied genotypes, except wild beetroot plants (Beta corolliflora Zoss.)were identified. As a result of the studies of SE2 gene, eight single nucleotide substitutions (3 T/C, 2 C/G, A/G, G/A, C/T) and three single nucleotide insertions (nucleotide A) were identified in plants of Shannon foreign hybrid compared to the resistant genotype. As for loci encoding protective proteins (regions of the second and seventh chromosomes) and involved in formation of resistance to Fusarium rot, SNPs were also found in domestic breeding materials and foreign hybrids. The Geneious Prime tool was used to analyze and align the sequenced sequences.