Identification of New Proteins and Potential Mitochondrial F1F0-ATPase Inhibitor Factor 1-Associated Mechanisms in Arabidopsis thaliana Using iTRAQ-Based Quantitative Proteomic Analysis

Cuiting Chen,Yiqing Meng,Mingfang Zhang,Zhongyuan Hu,Jinghua Yang

doi:10.3390/plants10112385

Cuiting Chen, Yiqing Meng + Show 3 more

Open Access

https://doi.org/10.3390/plants10112385

Copy DOI

Abstract

The mitochondrial synthesis of ATP makes a vital contribution to the growth and development of biological organisms, in which the enzyme mitochondrial F1F0-ATP synthase plays a pivotal role, in that it can either synthesize or hydrolyze cellular ATP. The finding of our previous study revealed that mitochondrial F1F0-ATPase inhibitor factor 1 (IF1) in Arabidopsis thaliana has a conserved function as an endogenous inhibitor affecting cellular energy status and plays an important role in plant growth and reproduction, particularly in fertility. In this study, to gain an insight into IF1-related traits, we performed isobaric tags for relative and absolute quantitation labeling analysis. In total, 67 of 4778 identified proteins were identified as differentially expressed proteins (DEPs; 59 up-regulated and 8 down-regulated) between wild-type and if1 mutant Arabidopsis thaliana seedlings. Gene ontology enrichment analysis revealed that these DEPs were the most significantly enriched in pathways such as “long-day photoperiodism, flowering,” “positive regulation of protein import into chloroplast stroma,” and “pollen sperm cell differentiation,” which are closely associated with reproductive development. Moreover, Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that photosynthesis was the pathway most significantly enriched with DEPs. Collectively, our results revealed a global shift in protein abundance patterns corresponding to AtIF1 mutation, entailing changes in the abundance of multiple key proteins and metabolic processes, which will provide a valuable proteomic foundation for future studies.

Highlights

In mitochondria, F1F0-ATPase synthesizes the energy-bearing compound, ATP plays a physiological pH-dependent role in ATP hydrolysis [1,2]
inhibitor factor 1 (IF1) inhibits the ATP hydrolysis activity of mitochondrial F1F0-ATPase when the impaired membrane potential is rectified, and ATP hydrolysis is not needed to ensure an adequate supply of ATP for cell physiological activity, which plays an important role in cell development [6,7]
To examine total protein changes in the protein profiles and to gain a global view of the cellular processes occurring in response to Arabidopsis thaliana IF1 (AtIF1) mutation, we performed comparative proteomics analysis using a multiplex Isobaric tags for relative and absolute quantitation (iTRAQ) technique in A. thaliana wild-type (WT) and T-DNA knockout if1 mutant 4-week-old seedlings

Summary

Introduction

F1F0-ATPase synthesizes the energy-bearing compound, ATP plays a physiological pH-dependent role in ATP hydrolysis [1,2]. IF1 inhibits the ATP hydrolysis activity of mitochondrial F1F0-ATPase when the impaired membrane potential is rectified, and ATP hydrolysis is not needed to ensure an adequate supply of ATP for cell physiological activity, which plays an important role in cell development [6,7]. We characterized IF1 function in Arabidopsis thaliana and established that Arabidopsis thaliana IF1 (AtIF1) affects the energy status of cells and plays important roles in growth and reproductive development, as mutation of this gene results in a decrease in dark-enhanced hypocotyl elongation and seed yield [11]. It is to be assumed that the molecular mechanisms and networks of IF1 in plant energy regulation are more complex than those in non-photosynthetic organisms

Methods

Results

Conclusion