Abstract
To isolate the microsatellites from the chromosomal DNA of the Korean cattle (Hanwoo) and to use those for the genetic selection, four bacteriophage genomic libraries containing the chromosomal DNA of six Hanwoo steers showing the differences in meat quality and quantity were used. Screening of the genomic libraries using 32 P-radiolabeled 5`-(CA)12-3' nucleotide as a probe, resulted in isolation of about 3,000 positive candidate bacteriophage clones that contain (CA)n-type dinucleotide microsatellites. After confirming the presence of microsatellite in each positive candidate clone by Southern blot analysis, the DNA fragments that include microsatellite and flanking sequences possessing less than 2 kb in size, were subcloned into plasmid vector. Results from the analysis of microsatellite length polymorphism, using twenty-two PCR primers designed from flanking region of each microsatellite DNA, demonstrated that 208 and 210 alleles of HW-YU-MS#3 were closely related to the economic traits such as marbling score, daily gain, backfat thickness and M. longissimus dorsi area in Hanwoo. Interestingly, HW-YU-MS#3 microsatellite was localized in bovine chromosome 17 on which QTLs related to regulation of the body fat content and muscle hypertrophy locus are previously known to exist. Taken together, the results from the present study suggest the possible use of the two alleles as a DNA marker related to economic trait to select the Hanwoo in the future. (Asian-Aust. J. Anim. Sci. 2004. Vol 17, No. 10 : 1329-1333)
Highlights
The Korean Cattle (Hanwoo), with their genetic sources, have been well-preserved for several thousand years because of the unique geographical situation in Korean peninsula
The major goal of genomic research in livestock has been focused on developing genetic map and identifying genes associated with important economic traits
Since there are several millions of microsatellites pertaining to bovine genome and they are highly polymorphic, via many of genomic researchs such as EST
Summary
The Korean Cattle (Hanwoo), with their genetic sources, have been well-preserved for several thousand years because of the unique geographical situation in Korean peninsula. The Hanwoo bacteriophage genomic library was used to screen the microsatellite DNA in Hanwoo chromosome with slight modifications of the standard protocol (PCR) for microsatellite was performed using 20 ng of Hanwoo genomic DNA as template with 1×PCR buffer, 1
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