Abstract
Using a Tn5 mutagenesis in a Sinorhizobium meliloti strain CXM 1-105, we obtained three mutants with increased symbiotic efficiency with alfalfa. Our study demonstrated Tn5 insertions labeled two chromosome genes encoding 5-adenosylhomocysteine methylthioadenosine deaminase (mutant T4) and a protein belonging to the GntR family of transcription regulators (mutant M3) and a thiamine biosynthetic gene thiC, localized at megaplasmide 2 (mutant T795). Mutant T795 along with high symbiotic efficiency exhibited a reduced ability to compete for nodulation with the parent strain CXM1-105.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
