Identification of Nepeta olgae Regel and phylogenetic status of some genera in subtribe Nepetinae (Lamiaceae) using DNA markers

V.V Chepinoga,Abdurashid Rakhmatov,Elena Nikitina,K.P Ma

doi:10.1051/bioconf/20213800087

V.V Chepinoga, Abdurashid Rakhmatov + Show 2 more

Open Access

https://doi.org/10.1051/bioconf/20213800087

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Abstract

The species level diversity is the reference unit for biodiversity accounting, should be systematized and include full information about the species. Reliable identification of any species is critical for a large-scale biodiversity monitoring and conservation. A DNA barcode is a DNA sequence that identifies a species by comparing the sequence of an unknown species with barcodes of a known species sequence database. Accurate identification of important plants is essential for their conservation, inventory. The species diversity assessing exampled on the subtribe Nepetinae (Lamiaceae) representatives, growing in Uzbekistan is given, using DNA barcoding method. The study was aimed to identify indigenous important plants with the nuclear (ITS) and plastid (matK, rbcL, trnL-F) genomes. This work demonstrates the phylogenetic relationships of some genera within the subtribe Nepetinae Coss. & Germ. (Lamiaceae), based on ITS locus gene. All results indicate that the DNA barcoding tool can be successfully used to reliably identify important plants, to inventory the botanical resources of Uzbekistan and to create a reference library of DNA barcodes. So, the combination of three-four locus gene is a good candidate for this approach.

Highlights

According to modern estimates, there are about 308 312 species of vascular plants on Earth [1]
It accounts over 4400 species of vascular plants, and includes a rare, relict species groups (10–12% of the flora of Uzbekistan), 378 taxa are considered as a national endemics, the study of which makes possible to assess the processes of speciation
The important and rare represents of the genera Nepeta, Dracocephalum, Lallemantia included in subtribe Nepetinae (Lamiaceae) growing in Uzbekistan were used as a research material

Summary

Introduction

There are about 308 312 species of vascular plants on Earth [1]. The major threat factors include habitat transformation, overexploitation of natural resources, the spread of invasive alien species, environmental pollution and climate change [2]. Flora of Uzbekistan is rich in plant species diversity. It accounts over 4400 species of vascular plants, and includes a rare, relict species groups (10–12% of the flora of Uzbekistan), 378 taxa are considered as a national endemics, the study of which makes possible to assess the processes of speciation. Rare and endangered species require a special attention, regular monitoring of their natural populations and comprehensive study by classical taxonomy methods, as well as molecular genetic methods. The rare important species of the subtribe Nepetinae Coss. Some genera that represent the subtribe Nepetinae in Uzbekistan are Nepeta L., Dracocephalum L., Drepanocaryum Pojark., Lophanthus Adans., Hyssopus L., Lallemantia Fisch.

Results and discussion

Taxa Nepeta olgae Regel

Identification number of Database

Salvia korolkovii*