Identification of MVOCs Produced by Coniophora puteana and Poria placenta Growing on WPC Boards by Using Subtraction Mass Spectra.

Abstract

Volatile fungal metabolites are responsible for various odors and may contribute to a “sick building syndrome” (SBS) with a negative effect on the heath of building. The authors have attempted to fill the research gaps by analyzing microbial volatile organic compounds (MVOCs) originating from representatives of the Basidiomycetes class that grow on wood-polymer composite (WPC) boards. WPCs have been analyzed as a material exposed to biodeterioration. Indoor air quality (IAQ) is affected by the increased use of WPCs inside buildings, and is becoming a highly relevant research issue. The emission profiles of MVOCs at various stages of WPC decay have been demonstrated in detail for Coniophora puteana and Poria placenta, and used to set the European industrial standards for wood-decay fungi. Differences in the production of MVOCs among these species of fungi have been detected using the thermal desorption-gas chromatography/mass spectrometry (TD-GC/MS) method. This study identifies the production of alcohols, aldehydes, ketones, carboxylic acids and other compounds during one month of fungal growth. The identified level of metabolites indicates a relation between the level of air pollution and condition of the WPC material, which may become part of IAQ quantification in the future. The study points to the species-specific compounds for representatives of brown and white-rot fungi and the compounds responsible for their odor. In this study, 1-Octen-3-ol was indicated as a marker for their active growth, which is also associated with SBS. The proposed experimental set-up and data analysis are a simple and convenient way to obtain emission profiles of MVOCs from microbes growing on different materials.