Abstract
Muscle cells were isolated from the longitudinal muscle layer of guinea pig and human jejunum and used to identify the muscarinic receptor subtype (M1 or M2) that mediates contraction. Single muscle cells were anchored to the ceiling of a minichamber and their contraction was measured in response to acetylcholine, alone and in combination with three muscarinic antagonists: atropine, 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP), and pirenzepine. Estimates of the inhibitory dissociation constants (Ki) were closely similar in human and guinea pig muscle cells (atropine 2.5–5 × 10−11 M. 4-DAMP 1.9–2.9 × 10 −10 M, and pirenzepine 8.2–9.5 × 10 −8 M). Thus, pirenzepine, a preferential M1 antagonist, was 1900–3280 times less potent than atropine and 279–500 times less potent than 4-DAMP. Comparative measurements on longitudinal muscle strips from guinea pig jejunum confirmed the greater potency of atropine and 4-DAMP relative to pirenzepine. Inactivation of muscarinic receptors on single muscle cells with dibenamine showed that only a small fraction of receptors was responsible for the response to acetylcholine. It was concluded that intestinal muscle cells contain a large reservoir of muscarinic M2 receptors that exhibit considerable spareness and heterogeneity.
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