Identification of mRNA and microRNA profiles in right ventricular dysfunction induced type II cardiorenal syndrome

Abstract

Abstract Background Type II Cardiorenal syndrome (CRS) induced by right ventricular (RV) dysfunction is a disease with high mortality but little attention. Its unique pathophysiology and molecular characteristics remains puzzled. This study is aimed to investigate the transcriptomic expression profile of circRNAs, miRNAs and mRNAs, and identify the potential therapeutic targets that regulate molecular signaling networks in RV dysfunction-induced type II CRS. Methods Male C57BL/6 mice receiving pulmonary artery constriction were used to establish RV dysfunction model. The expression profiles of mRNAs, miRNAs and circRNAs in RV and kidney tissues were obtained by whole transcriptome sequencing. Bioinformatics analyses were performed, including Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and co-expression network analysis. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to confirm the results of transcriptome sequencing. Findings A total of 741 and 86 differentially expressed (DE) mRNAs, 159 and 29 DEmiRNAs and 233 and 104 DEcircRNAs were found in RV and kidney tissue, respectively. Pairs of miRNA-mRNA were established. GO and KEGG analysis displayed a significant correlation with proliferation signaling pathways and metabolic pathways. Then the circRNA-miRNA-mRNA network related to proliferation and metabolic pathways were established. We identified hub circRNA-miRNA-mRNA pairs affected the fibrotic, proliferative and metabolic pathways significantly. Interpretation These findings indicate that the function of ceRNA networks play an important role in RV dysfunction induced type II CRS. Therefore, these circRNA-miRNA-mRNA pairs should be potential targets for treatment of this disease. Figure 1 Funding Acknowledgement Type of funding source: None