Abstract
Y73 is a progeny of asymmetric somatic hybridization between Oryza sativa cv. Dalixiang and the wild rice species Oryza meyeriana. Inoculation with a range of strains of Xanthomonas oryzae pv. oryzae showed that Y73 had inherited a high level of resistance to rice bacterial blight (BB) from its wild parent. An F2 population of 7125 individuals was constructed from the cross between Y73 and a BB-susceptible cultivar IR24. After testing 615 SSR and STS markers covering the 12 rice chromosomes, 186 markers were selected that showed polymorphism between Y73 and IR24. Molecular markers linked to the BB resistance genes in Y73 were scanned using the F2 population and the polymorphic markers. The SSR marker RM128 on chromosome 1, the STS marker R03D159 on chromosome 3 and the STS marker R05D104 on chromosome 5 were found to be linked to the rice BB resistance genes in Y73.
Highlights
Bacterial blight (BB) of rice, caused by Xanthomonas oryzae pv. oryzae, is one of the most important bacterial diseases worldwide
The hybrid progeny Y73 possessed a high level of the wild rice BB resistance (Fig. 1)
Because Dalixiang and Y73 were japonica, an indica cultivars IR24 was selected as another parent of the segregating population to produce marker polymorphism between the parents
Summary
Bacterial blight (BB) of rice, caused by Xanthomonas oryzae pv. oryzae, is one of the most important bacterial diseases worldwide. Oryzae, is one of the most important bacterial diseases worldwide It is responsible for heavy losses of global rice production, generally in the range 20% to 30% but as high as 50% in years when the disease is Received July 11, 2015; accepted September 20, 2015. Somatic hybridization through the fusion of two somatic protoplasts has already been used to overcome sexual incompatibility and produce inter-species hybrids, and this technique has been improved as asymmetric somatic hybridization to reduce the co-introduction of negative traits from the donor species[5]. Using this breeding technique, we have introduced BB resistance from O. meyeriana into a japonica rice cv. Dalixiang, which has good agronomic characters but is susceptible to BB[3,6]
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