Identification of miRNA-mRNA crosstalk in laryngeal squamous cell carcinoma.

Abstract

The aim of the present study was to elaborate the underlying pathogenesis of laryngeal squamous cell carcinoma (LSCC). Micro (mi) RNA and messenger (m) RNA expression profiling of patients with LSCC were downloaded from The Cancer Genome Atlas (TCGA) database. Differentially expressed miRNAs (DEMIs) and differentially expressed mRNAs (DEMs) were identified in LSCC compared to normal control tissues. The DEMs targeted by DEMIs were identified and the negative correlation between DEMs and DEMIs was subjected to visualization. The potential functions of DEMs targeted by DEMIs were annotated in Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A total of 663 dysregulated DEMs (449 upregulated and 214 downregulated) and 33 DEMIs (24 upregulated and 8 downregulated) were identified in LSCC compared with normal controls. 502 negative correlations between DEMIs and DEMs were identified and subjected to construct interaction network. In the network, hsa‑miR‑486, ‑34c, ‑206 and ‑182 had the highest connectivity with DEMs, and respectively regulated 39, 33, 28 and 27 DEMs. DEMs targeted by DEMIs were significantly enriched in signal transduction, actin binding and extracellular region of GO terms and focal adhesion and extracellular matrix‑receptor interaction of KEGG pathways. The present study may provide valuable information for understanding the potential oncogenesis mechanism in LSCC and provide the foundation work for diagnosis biomarkers and therapeutic targets for LSCC.