Identification of miRNA Signature Associated With Erectile Dysfunction in Type 2 Diabetes Mellitus by Support Vector Machine-Recursive Feature Elimination.

Haibo Xu,Baoyin Zhao,Hong Qiao,Wei Zhong,Peng Teng

doi:10.3389/fgene.2021.762136

Abstract

Diabetic mellitus erectile dysfunction (DMED) is one of the most common complications of diabetes mellitus (DM), which seriously affects the self-esteem and quality of life of diabetics. MicroRNAs (miRNAs) are endogenous non-coding RNAs whose expression levels can affect multiple cellular processes. Many pieces of studies have demonstrated that miRNA plays a role in the occurrence and development of DMED. However, the exact mechanism of this process is unclear. Hence, we apply miRNA sequencing from blood samples of 10 DMED patients and 10 DM controls to study the mechanisms of miRNA interactions in DMED patients. Firstly, we found four characteristic miRNAs as signature by the SVM-RFE method (hsa-let-7E-5p, hsa-miR-30 days-5p, hsa-miR-199b-5p, and hsa-miR-342–3p), called DMEDSig-4. Subsequently, we correlated DMEDSig-4 with clinical factors and further verified the ability of these miRNAs to classify samples. Finally, we functionally verified the relationship between DMEDSig-4 and DMED by pathway enrichment analysis of miRNA and its target genes. In brief, our study found four key miRNAs, which may be the key influencing factors of DMED. Meanwhile, the DMEDSig-4 could help in the development of new therapies for DMED.

Highlights

Erectile dysfunction (ED) refers to the persistent or repeated failure of men to achieve and/or maintain penile erection for satisfactory sexual activity
Statistical analysis was performed for the Diabetic mellitus erectile dysfunction (DMED) group and the diabetes mellitus (DM) group, including age, diabetes duration, Body mass index (BMI), fasting plasma glucose, glycated hemoglobin, total cholesterol, triglyceride, testosterone, thyroid stimulating hormone, serum creatinine, carbamide, alanine aminotransferase, aspartate aminotransferase
Wang et al found that upregulation of miR-320 was miRNA Signature for Diabetic Erectile Dysfunction associated with impaired angiogenesis in diabetes (Wang et al, 2009)

Summary

Introduction

Erectile dysfunction (ED) refers to the persistent or repeated failure of men to achieve and/or maintain penile erection for satisfactory sexual activity. As a common and the most neglected complication of diabetes (Zhao et al, 2020; Long et al, 2021; Yang et al, 2021), diabetic mellitus erectile dysfunction (DMED) is an important factor affecting psychological well-being, spousal relationship and family life (Malavige and Levy, 2009). The massive research indicated patient of T2MD incidence ED was significantly higher than that of the health. 66.3% is T2MD among of the data (Kouidrat et al, 2017; Cheng et al, 2018; Zagidullin et al, 2019; Zhu et al, 2021a). DMED is considered as an alternative marker for diabetes and cardiovascular disease, and is the primary feature of diabetes. DMED has a multifactorial pathological process that can occur simultaneously with cardiovascular disease, neuropathy, and depression. How to effectively intervene in DMED has become an urgent problem in the global medical community

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