Abstract
There is increasing evidence that the deregulation of microRNAs (miRNAs; miRs) contributes to tumorigenesis. Previous studies have shown that miR‑195 is downregulated in various types of cancer. The present study aimed to investigate the function and expression levels of miR‑125b. Results of qPCR revealed that miR‑195‑3p, the mature sequence of miR‑195, was upregulated in renal cell carcinoma (RCC) tissues and cell lines (786‑O, 769P and ACHN). This indicated that the function and role of miR‑195‑3p may differ in different types of tumor. To assess the function of miR‑195‑3p in RCC cell lines, cell proliferation was examined using MTT and CCK‑8 assays, mobility was assessed using a cell scratch assay, Transwell migration assay and invasion assay, and apoptosis was examined using flow cytometry. These assessments were also performed in cells with upregulated or downregulated miR‑195‑3p via transfection with synthesized miR‑195‑3p mimic or inhibitor. The results revealed that the overexpression of miR‑195‑3p promoted 786‑O and ACHN RCC cell proliferation, migration and invasion, and inhibited cell apoptosis, whereas the downregulation of miR‑195‑3p suppressed cell proliferation, migration and invasion, and induced cell apoptosis. These results indicated that miR‑195‑3p was associated with the tumorigenesis of RCC, with further investigations to focus on the pathway and use of miR‑195‑3p as a clinical biomarker for RCC.
Highlights
Renal cell carcinoma (RCC), a common malignant tumor originating from renal tubular epithelial cells, is the mostKey words: renal cell carcinoma, microRNA, microRNA‐195‐3p, tumor markers common type of renal cancer and the third most common type of urological cancer, accounting for 2‐3% of all adult malignancies according to a survey conducted in 2013 in the USA [1,2]
The ratios of expression of miR‐195‐3p in 31 paired RCC tissue samples are shown in Fig. 1A, which showed miR‐195‐3p was upregulated in 21 tissue samples
The results demonstrated that the expression levels of miR‐195‐3p in 786‐O, 769P and ACHN cells was 33.21, 3.90‐ and 3.31‐fold higher, compared with the expression levels in 293T cells, respectively (Fig. 1C)
Summary
Renal cell carcinoma (RCC), a common malignant tumor originating from renal tubular epithelial cells, is the most. The function of miRNAs as oncogenes or tumor suppressors depends on the target gene they regulate. Previous studies have demonstrated that miRNAs are associated with various cellular processes, including proliferation, apoptosis, differentiation and stress response [6,10]. Previous miRNA microarray chip analysis of RCC showed that miR‐195‐3p, the mature sequence of miR‐195 termed miR‐195, was upregulated [15], which revealed that the role of miR‐195‐3p in RCC may be different, compared with other tumors. The present study performed reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) analysis to detect the expression level of miR‐195 in RCC tissues and cell lines, and the investigated the role of miR‐195‐3p in RCC tumorigenesis by performing cell proliferation, mobility and apoptotic assays
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