Abstract
Background Pinus tecunumanii has displayed good performance in tropical regions of Brazil and showed high potential for commercial exploitation. Embrapa Forestry and its partners own many of the species seed production areas. In spite of its importance, the majority of P. tecunumanii germplasm collections remain still genetically uncharacterized. Thus identifying genetic markers is an important tool to genetically characterize these collections.We describe the initial steps to develop microsatellites for Pinus tecunumanii by enriched library construction with the ultimate goal of characterizing accessions of the germplasm collections of EMBRAPA.
Highlights
Pinus tecunumanii has displayed good performance in tropical regions of Brazil and showed high potential for commercial exploitation
The genomic DNA of P. tecunumanii was digested with AFAI and enriched in (CT)8 and (GT)8 repeats
Enriched fragments were amplified by polymerase chain reaction (PCR), connected to a pGEM T-easy vector and transformed into competent XL1- blue Escherichia coli cells
Summary
Background Pinus tecunumanii has displayed good performance in tropical regions of Brazil and showed high potential for commercial exploitation. Embrapa Forestry and its partners own many of the species seed production areas. In spite of its importance, the majority of P. tecunumanii germplasm collections remain still genetically uncharacterized. Identifying genetic markers is an important tool to genetically characterize these collections.We describe the initial steps to develop microsatellites for Pinus tecunumanii by enriched library construction with the ultimate goal of characterizing accessions of the germplasm collections of EMBRAPA.
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