Abstract
MicroRNAs (miRNAs) are small, non-coding and regulatory RNAs about 20 to 24 nucleotides long. Their conserved nature among the various organisms makes them a good source of new miRNAs discovery by comparative genomics approach. This study resulted in 34 miRNAs belonging to 21 families in ecological model plant Mimulus. All the miRNA families (mir 156, 157, 159, 160, 164, 172, 319, 393, 395, 397, 399, 400, 403, 417, 419, 472, 782, 854, 858, 867 and 2112) are found for the first time in Mimulus. The MIR-399 is found as precursor miRNA cluster with 4 mature sequences. All 34 miRNA precursors form stable minimum free energy stem loop structure as their orthologues form and the mature miRNAs reside in the stem portion of the stem loop structure. Twenty eight are from Mimulus guttatus and six miRNAs belong to Mimulus lewisii. Their targets consist of dihy-droflavonol-4-reductase, cycloidea-like protein, DNA-directed RNA polymerase II, maturase (matR) and transcription factors like; squamosa-promoter binding, MYB, palmate-like pentafoliata 1.
Highlights
A special RNaseIII-like endonuclease, Dicer-like enzyme (DCL) in plants involved to process the mature miRNA production [9], that mostly integrate the mature miRNA into the RNA induced silencing complex (RISC) [8]
Twenty eight are from Mimulus guttatus and six miRNAs belong to Mimulus lewisii
The stem portion of the hairpin checked for the mature sequences with at least 12 base pairs involved in Watson-Crick or G/U base pairing between the mature miRNA and the opposite strand
Summary
It is called as the monkey-flowers and musk-flowers. It belongs to the family Phrymaceae consist of about 150 species [1]. MicroRNAs (miRNAs) are non-coding, endogenous, small RNAs about 20 - 24 nucleotide long [3]. They are conserved in plants and animals [4,5] and play vital role in post transcriptional gene regulation. The conserved nature of these miRNAs becomes a logical approach for identification of new orthologues by comparative genomics in other species [21,22]. Twenty eight are from Mimulus guttatus and six miRNAs belong to Mimulus lewisii
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