Identification of microbiota in peri-implantitis pockets by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Hwey-Chin Yeh,Jang-Jih Lu,Shih-Cheng Chang,Mao-Cheng Ge

doi:10.1038/s41598-018-37450-5

Hwey-Chin Yeh, Jang-Jih Lu + Show 2 more

Open Access

https://doi.org/10.1038/s41598-018-37450-5

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Abstract

The purpose of this study was to identify the microbial communities that colonize peri-implantitis pockets using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Subjects having at least one implant with peri-implantitis, no diabetes, and not taking antibiotics in the previous 3 months were selected. Peri-implantitis was defined when surrounding bone loss ≥0.5 mm and bleeding on probing was found. Microbial samples were collected from peri-implantitis pockets using paper points. After incubation and isolation, the colonies were analyzed by MALDI-TOF MS. A total of 126 isolates were cultivated and identified from 12 samples, in identification rates of 82.5% at the species level and 12.72% at the genus level. Although the compositions were highly variable, major habitants in different peri-implant pockets could be identified. Among them the most distinguished were Neisseria flavescens (87%), Streptococcus constellatus (56%), Slackia exigua (46%), Streptococcus intermedius (45%), Fusobacterium nucleatum (45%) and Gemella morbillorum (43%). This preliminary study provides comprehensive and reliable data for future study designs involving MALDI-TOF MS and peri-implantitis in a more specific, easy, rapid and economical way. MALDI-TOF MS could be a new clinical method to evaluate and monitor oral microbiota associated with the disease.

Highlights

The purpose of this study was to identify the microbial communities that colonize peri-implantitis pockets using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)
Microorganisms are best identified using polymerase chain reaction (PCR) amplification of conserved regions of the 16S ribosomal RNA gene followed by clone library construction, which allows for the detection of previously uncultivated and unknown bacteria[9]
MALDI-TOF-MS has emerged as a potential tool for microbial identification and diagnosis and has barely been used for bacterial identification in peri-implantitis

Summary

Introduction

The purpose of this study was to identify the microbial communities that colonize peri-implantitis pockets using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Diagnosis of peri-implantitis is made in a similar way to that of periodontitis It is detected both clinically and radiographically, including bleeding on probing and loss of supporting bone. Microbiologic methods used to study the presence of microorganisms in peri-implantitis sites seem to influence results of microbial profile studies Microbiological results by both culture and checkerboard analysis only detect specific target bacteria. Microorganisms are best identified using polymerase chain reaction (PCR) amplification of conserved regions of the 16S ribosomal RNA (rRNA) gene followed by clone library construction, which allows for the detection of previously uncultivated and unknown bacteria[9] It is often expensive and time consuming, and quantitative results might be misleading because live and dead bacteria cannot be distinguished. Correspondence and requests for materials should be addressed to www.nature.com/scientificreports/

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