Identification of metal toxicity in sewage sludge leachate

Abstract

Sewage sludge is a source of organic matter and nutrients with the potential for being used as a fertilizer. However, metals in sewage sludge might accumulate in soil after repeated sludge applications, and metal concentrations might reach concentrations that are toxic to microorganisms, soil organisms and/or plants. This toxicity might change with time due to kinetic factors or abiotic factors such as freezing, drying or rainfall. The objective of this study was to determine toxicity of sewage sludge leachate from a lysimeter with 50 cm of sludge applied. Attempts were also made to identify the cause of toxicity of the sludge leachate by toxicity identification and evaluation (TIE) techniques. Sludge leachate was collected monthly during 1 experimental year (August 2001 to August 2002). Metal concentrations were analysed, and the toxicity was determined with Daphnia magna (48-h immobility). The effect of EDTA or sodium thiosulphate addition, filtration through a CM-resin or a Millex-resin on toxicity was also tested. The results showed that toxicity of the sludge leachate apparently varied during the year, and that filtration through the CM-resin reduced most of the toxicity followed by the addition of EDTA. None of the other treatments reduced the toxicity of the sludge leachate. This indicated that one or more metals were responsible for the observed toxicity. Further calculations of toxic units (TU) suggested that Zn contributed most to the toxicity. Results also indicated that Ca concentrations in the sludge leachate reduced the toxicity of Zn.