Identification of Metal‐Associated Proteins in Cells by Using Continuous‐Flow Gel Electrophoresis and Inductively Coupled Plasma Mass Spectrometry

Abstract

Metal detector: The title strategy provides a new way for rapid profiling of the metal-associated proteins on a proteome- and metallome-wide scale. The technique was applied to identify protein targets of a bismuth-based drug in Helicobacter pylori and to study the metal binding of the protein SlyD, which is a member of the FK506-binding protein family, in E. coli.