Identification of marine bacteria HPP.4A and HPP.T13 and its anticancer activity against P388 murine leukaemia cell

Abstract

Sea cucumber has been widely studied as a source of bioactive compounds with various biological activities such as antibacterial, antifungal, antioxidant, and anticancer. However, there are a few studies have reported on the biological activity of its associated bacteria. The purpose of this study were to determine the potential of sea cucumber associated bacteria from Panjang Island, Jepara, Central Java, Indonesia as a natural source of anticancer compounds and identify the prospective isolates through DNA barcoding. Bacteria HPP.4A and HPP.T13 were isolated from the gut of sea cucumber Holoturia atra. The bacteria were cultivated in three different media (A3, A11, and A16) then extracted using 1-butanol with maceration method. Cytotoxic assay of each extract was conducted against P388 murine leukaemia cell. Bacteria HPP.4A and HPP.T13 were identified through molecular approach as Sallinicoccus roseus and Sphingobium yanoikuyae with 99.73% similarity. The strongest anticancer activity was showed by Sallinicoccus roseus extract which cultivated in A11 medium while Sphingobium yanoikuyae extract in A3 medium.