Abstract
Channel catfish (Ictalurus punctatus) is an important aquaculture species worldwide. Although studies of the sex determination mechanism of channel catfish began during the 1980s, the mechanism and sex-specific genes remain unclear. In this study, based on the results of sex-related quantitative trait locus (QTL) mapping and targeted next-generation sequencing (NGS) technique, 13 male-specific single nuclear polymorphisms (SNPs) were identified in the coding region of the zbtb38 gene of channel catfish by targeted next-generation sequencing. Among them, six substitutions resulted in changes in the amino acid coding in zbtb38 on both the X and Y chromosomes. It is speculated that zbtb38 on the Y chromosome has an important role in testicular differentiation and is an important candidate gene for sex determination in channel catfish. Subsequently, male-specific primers were designed according to male-specific nucleotides, which amplified an expected band from males but not from females. Thus, a rapid and effective method was successfully established to identify the genetic sex of channel catfish. Our study contributes to the study of the sex determination mechanisms and development of mono-sex populations in channel catfish.
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