Abstract
BackgroundSpotted wilt caused by tomato spotted wilt virus (TSWV) is one of the major peanut (Arachis hypogaea L.) diseases in the southeastern United States. Occurrence, severity, and symptoms of spotted wilt disease are highly variable from season to season, making it difficult to efficiently evaluate breeding populations for resistance. Molecular markers linked to spotted wilt resistance could overcome this problem and allow selection of resistant lines regardless of environmental conditions. Florida-EPTM ‘113’ is a spotted wilt resistant cultivar with a significantly lower infection frequency. However, the genetic basis is still unknown. The objective of this study is to map the major quantitative trait loci (QTLs) linked to spotted wilt resistance in Florida-EPTM ‘113’.ResultsAmong 2,431 SSR markers located across the whole peanut genome screened between the two parental lines, 329 were polymorphic. Those polymorphic markers were used to further genotype a representative set of individuals in a segregating population. Only polymorphic markers on chromosome A01 showed co-segregation between genotype and phenotype. Genotyping by sequencing (GBS) of the representative set of individuals in the segregating population also depicted a strong association between several SNPs on chromosome A01 and the trait, indicating a major QTL on chromosome A01. Therefore marker density was enriched on the A01 chromosome. A linkage map with 23 makers on chromosome A01 was constructed, showing collinearity with the physical map. Combined with phenotypic data, a major QTL flanked by marker AHGS4584 and GM672 was identified on chromosome A01, with up to 22.7 % PVE and 9.0 LOD value.ConclusionA major QTL controlling the spotted wilt resistance in Florida-EPTM ‘113’ was identified. The resistance is most likely contributed by PI 576638, a hirsuta botanical-type line, introduced from Mexico with spotted wilt resistance. The flanking markers of this QTL can be used for further fine mapping and marker assisted selection in peanut breeding programs.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-016-0435-9) contains supplementary material, which is available to authorized users.
Highlights
Spotted wilt caused by tomato spotted wilt virus (TSWV) is one of the major peanut (Arachis hypogaea L.) diseases in the southeastern United States
Expression of spotted wilt disease in peanut is highly variable depending on the disease pressure which varies by location and season
Phenotypic distribution results in this study indicated that disease pressure at North Florida Research and Education Center (NFREC) was higher than Plant Science Research and Education Unit (PSREU)
Summary
Spotted wilt caused by tomato spotted wilt virus (TSWV) is one of the major peanut (Arachis hypogaea L.) diseases in the southeastern United States. Occurrence, severity, and symptoms of spotted wilt disease are highly variable from season to season, making it difficult to efficiently evaluate breeding populations for resistance. Florida-EPTM ‘113’ is a spotted wilt resistant cultivar with a significantly lower infection frequency. The objective of this study is to map the major quantitative trait loci (QTLs) linked to spotted wilt resistance in Florida-EPTM ‘113’. Peanut production is significantly affected by spotted wilt disease caused by Tomato spotted wilt virus (TSWV) (genus Tospovirus, family Bunyaviridae), in the United States. In 1997, the production losses due to spotted wilt in Georgia were estimated to be approximately $40 million [3]. The typical symptoms of spotted wilt on peanuts are yellowing, stunting, concentric ringspots, chlorosis, and necrosis of various sizes and shapes on leaflets [4]
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