Abstract
OSCA (Hyperosmdality-gate calcium-permeable channels) is a calcium-permeable cation channel protein. By participating in sensing changes in extracellular osmotic potential, it induces an increase in Ca 2+ concentration, regulates osmotic potential, and plays an important role in hypertonic stress induction. However, there are few studies on the OSCA genes of poplar. In this study, 16 OSCA genes sequences were identified from Populus tomentosa genome, and renamed them according to their distribution on the chromosomes. Based on phylogenetic tree analysis, the PtOSCAs genes were divided into 4 groups, and the OSCA genes in each group had similar gene structures. Two repetitive events were identified between poplar OSCA family genes, one tandem repetitive event involving 3 genes and a fragment repetitive event involving 2 genes, and the Ka/Ks value of each pair of genes was below 1. Therefore, it can be seen that the OSCA family genes of poplar have a purification effect during evolution. By analyzing the expression of PtOSCAs gene under low and high concentration salt stress, it is found that there are the following three types: Type I, where the expression level increases under both low and high concentration salt stress; Type II, at low and high concentration salt stress, the expression level decreased under high concentration salt stress; Type Ⅲ, the expression level showed an upward trend under low concentration salt stress, but the expression level showed a downward trend under high concentration salt stress. This study provides a reference for the functional analysis of PtOSCAs gene and the genetic improvement of poplar.
Highlights
By analyzing the expression of PtOSCAs gene under low and high concentration salt stress, it is found that there are the following three types: Type I, where the expression level increases under both low and high concentration salt stress; Type II, at low and high concentration salt stress, the expression level decreased under high concentration salt stress; Type III, the expression level showed an upward trend under low concentration salt stress, but the expression level showed a downward trend under high concentration salt stress
The results showed that 16 PtOSCAs genes were located on chromosomes
The results showed that the molecular weight range of PtOSCAs gene was concentrated in 56.32~94.89 kD, and the average molecular weight was 83.97 kD
Summary
There are many stress effects (such as salt-alkali, high temperature and drought) in nature, which will adversely affect the growth and development of plants, and even lead to plant death. The study of Ca2+ pathway is of great significance to clarify plant response to stress and regulate plant stress resistance. Plant Gene and Trait 2022, Vol., No.1, 1-10 http://genbreedpublisher.com/index.php/pgt this family, all of which have DUF221 domain related to plant stress response (Yuan et al, 2014). 10 differentially expressed OsOSCA genes were induced by osmotic stress (ABA, NaCl, PEG), among which OsOSCA3.1 was identified to be involved in early drought response OsERD4 gene (Rai et al, 2012; Yuan et al, 2014). OsOSCAI. in rice, AtOSCAI. in Arabidopsis thaliana and AtOSCA1.4 in wheat all belong to the same family, which are directly affected by related plant traits such as wheat yield, grain number per spike, apical sterility (Lü, 2015). There are 13 GmOSCAs genes in soybean, which are directly affected by salt-alkali stress and drought response (Li et al, 2017). It can be found that this gene is mainly related to abiotic stress, but the correlation between OSCA gene and abiotic stress in poplar has not been reported
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