Abstract
Detection of low abundance proteins always possesses challenges even with the currently available proteomics technologies. We aimed to evaluate the low abundance differentially expressed proteins in colorectal cancerous tissues as compared to colorectal normal tissues. Protein separation was carried out by using the coupling methods of hydroxyapatite chromatography and SDS-PAGE followed by mass spectrometry analysis. Five of the membrane associated low abundance proteins, namely uncharacterized protein C5orf4, coiled-coil domain containing protein 152, DnaJ homolog subfamily C member 22, tumor suppressor candidate 3 and leucine-rich repeat transmembrane neuronal protein 4 that previously only reported at the genome level were identified. The proteins were isolated from the cancerous tissues as either up-regulated or down-regulated proteins as compared to normal tissues and therefore may play important roles in cancer development.
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