Abstract
BackgroundMineral nutrients play a crucial role in the biochemical and physiological functions of biological systems. The enhancement of seed mineral content via genetic improvement is considered as the most promising and cost-effective approach compared alternative means for meeting the dietary needs. The overall objective of this study was to perform a GWAS of mineral content (Ca, K, P and S) in seeds of a core set of 137 soybean lines that are representative of the diversity of early maturing soybeans cultivated in Canada (maturity groups 000-II).ResultsThis panel of 137 soybean lines was grown in five environments (in total) and the seed mineral content was measured using a portable x-ray fluorescence (XRF) spectrometer. The association analyses were carried out using three statistical models and a set of 2.2 million SNPs obtained from a combined dataset of genotyping-by-sequencing and whole-genome sequencing. Eight QTLs significantly associated with the Ca, K, P and S content were identified by at least two of the three statistical models used (in two environments) contributing each from 17 to 31% of the phenotypic variation. A strong reproducibility of the effect of seven out these eight QTLs was observed in three other environments. In total, three candidate genes were identified involved in transport and assimilation of these mineral elements.ConclusionsThere have been very few GWAS studies to identify QTLs associated with the mineral element content of soybean seeds. In addition to being new, the QTLs identified in this study and candidate genes will be useful for the genetic improvement of soybean nutritional quality through marker-assisted selection. Moreover, this study also provides details on the range of phenotypic variation encountered within the Canadian soybean germplasm.
Highlights
Mineral nutrients play a crucial role in the biochemical and physiological functions of biological systems
These results demonstrated that the ED-XDF was appropriate for the quantification of Ca, P and K content in soybean seeds
Phenotypic variation and correlations among traits The concentrations of Ca, K, P and S on a set of 137 soybean lines grown on two sites in 2013 were estimated using an ED-x-ray fluorescence (XRF) device
Summary
Mineral nutrients play a crucial role in the biochemical and physiological functions of biological systems. The enhancement of seed mineral content via genetic improvement is considered as the most promising and cost-effective approach compared alternative means for meeting the dietary needs. The enhancement of seed mineral nutrient content via genetic improvement is considered as the most promising and cost-effective approach to ensure that the dietary needs of consumers are met. ED-XRF has been used successfully to assess Ca, K, P and S concentrations in soybean [8, 9], in cacao [7] and in pea seeds [10] In these studies, measurements obtained via spectroscopy were consistent with previous studies using more common but costly analytical methods. These studies have shown that mineral element content can span a large range of values in plants and that this content is determined both by genetic and environmental factors [2, 4, 11,12,13]
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