Abstract
BackgroundPrimary open angle glaucoma (POAG) is a multifactorial disorder characterized by a progressive permanent degeneration of retinal ganglion cell (RGCs) death. An increasing number of studies have suggested that long noncoding RNAs (lncRNAs) have the ability to regulate gene expression; however, thus far, the mechanisms and functions of lncRNAs in the development of POAG are still unclear.MethodsUsing the data from Gene Expression Omnibus (GEO), differentially expressed lncRNAs and differentially expressed mRNAs between POAG patients and controls were identified. Then, the lncRNA–miRNA–mRNA competing endogenous RNA (ceRNA) network was constructed, and the key lncRNAs in POAG were identified. A Gene Ontology (GO) analysis and a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to assess the enriched biological functions of mRNA in the ceRNA network.ResultsDuring this study, a POAG-related ceRNA network with 37 miRNA nodes, 248 lncRNA nodes, 178 mRNA nodes, and 1985 edges was constructed. In addition, four lncRNAs (DNAJC27-AS1, AF121898, OIP5-AS1, and SNX29P2) were established as hub RNAs in this ceRNA network. The functional assay showed that 18 GO terms and 17 pathways were enriched.ConclusionThis study provides novel insights into the lncRNA-related ceRNA network in POAG, and the four lncRNAs were identified in the development of POAG.
Highlights
Primary open angle glaucoma (POAG) is a multifactorial disorder characterized by a progressive permanent degeneration of retinal ganglion cell (RGCs) death
The heat maps of Long noncoding RNA (lncRNA), messenger RNA (mRNA), and all RNAs showed the differences between POAG patients and the control group (Fig. 1)
248 POAGspecific lncRNAs that putatively targeted 37 POAGspecific miRNAs and the comparisons of 37 POAGspecific miRNAs and 178 POAG -specific mRNAs were involved in the competing endogenous RNA (ceRNA) network
Summary
Primary open angle glaucoma (POAG) is a multifactorial disorder characterized by a progressive permanent degeneration of retinal ganglion cell (RGCs) death. An increasing number of studies have suggested that long noncoding RNAs (lncRNAs) have the ability to regulate gene expression; far, the mechanisms and functions of lncRNAs in the development of POAG are still unclear. Primary open angle glaucoma (POAG), a frequent type of glaucoma worldwide, is a complex characterized by the progressive permanent degeneration of retinal ganglion cell (RGCs) death and distinctive visual field loss [1, 2]. A non-coding RNA (ncRNA) is an RNA molecule that is not translated into a protein. Long noncoding RNA (lncRNA) is a typical ncRNA with a non-protein-coding function exceeding 200 nucleotides.
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