Identification of LL and Meso-diaminopimelic acid (DAP) of Actinomycete strains and Its Secondary Metabolite Production

Abstract

Streptomyces and Nocardia is a genus of actinomycetes group which has some similar characteristic. Although all of them have the ability to produce antibiotics but almost of Nocardia is pathogenic. The objective of this study is analyzed the two strains of actinomycetes and determine the optimum conditions of production of secondary metabolites using Streptomyces sp. Identification to distinguish the two strains (TP2 and TP5) by analyzing LL and meso-DAP on their cell walls. Optimization of the fermentation process using one factor at one time (OFAT) method that conducted at temperature of 26-40 0C, pH 5-8 and 120-180rpm for 48hours in a fermentor working volume 1 L. Antibacterial activity test performed by the agar diffusion method against resistant pathogenic bacteria. The results showed Streptomyces sp. TP2, contain any form of LL-DAP and Nocardia sp. TP5, and contain any form of meso-DAP as its main component. Optimization of antibiotic production is reached after fermentation be held for 42hours at a temperature of 30 0C, pH 7 and 180rpm with antibacterial activity against methicillin-sensitive Staphylococcus aureus, methicillin resistant Staphylococcus aureus, methicillin resistant coagulase negative Staphylococcus, vancomycin resistant Enterococcus, and Escherichia coli.