Abstract
The main aim of this work was to determine the principal lipid classes and compositions of fatty acids (FFAs) in triacylglycerols (TAGs) and phospholipids in adult shrimps Crangon crangon from coastal areas of the Gulf of Gdansk (Baltic Sea, Poland). High performance liquid chromatography with a laser light-scattering detector (HPLC-LLSD) was used to separate the lipid classes. The FFA composition was determined with gas chromatography-mass spectrometry (GC-MS) and matrix-assisted laser-desorption time-of-flight mass spectrometry (MALDI-TOF-MS). The following lipid classes were separated using HPLC-LLSD: TAGs, FFAs, sterols (ST) and polar lipids (PL). The HPLC-LLSD analysis showed the dominance of sterols, which were missing from MALDI-TOF-MS, sterols made up 2.5 mg g-1 of the total lipids on the basis of this analysis. TAGs and FFAs were the next most abundant lipid classes, accounting 1.9 mg g-1 and 2.0 mg g-1 of the total lipids, respectively.
Highlights
Lipids include fatty acids, their derivatives, and substances biosynthetically or functionally related to these compounds.[1]
Four fractions were obtained as a result of the HPLC-LLSD separations: TAGs (f1), FFAs (f2), sterols (f3) and polar lipids (PL) (f4) (Figure 1)
Sterols were the dominant class of lipids and accounted 2.5 mg g-1 of the total lipids
Summary
Their derivatives, and substances biosynthetically or functionally related to these compounds.[1] They form dynamic bilayer membranes and are the main source of energy.[2,3,4] Fatty acids are basic structural components of complex lipids, such as phospholipids and glycolipids. Their derivatives are hormones and intercellular informers, and they interface with proteins as their lipophilic modifiers. Those FFAs that cannot be synthesized by mammals are called essential fatty acids (EFAs), they are of Identification of Lipid Components in the Abdominal Muscle of Fall-Caught Crangon crangon
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