Identification of LIG1 and LIG3 as prognostic biomarkers in breast cancer.

Chunying Shi,Xinyu Liu,Siqi Song,Lingjun Feng,Haiqing Hou,Bo Chen,Lin Sun,Zhipeng Sun

doi:10.1515/med-2021-0388

Abstract

DNA ligase (LIG) plays a key role in connecting the 3′-OH end of a DNA strand to the 5′-P end of another DNA strand, resulting in the formation of a phosphodiester bond. It has been reported that LIGs (including LIG1, LIG3 and LIG4) play important roles in the occurrence and progression of many cancers. However, the role of LIGs in breast cancer (BC) is still unclear. In this study, we aim to reveal the expression level, function, and prognostic value of LIGs in BC. Bioinformatic tools were used to study the expression level, potential function and prognostic value of LIG1 and LIG3 in BC patients. ENCORI was used to predict microRNAs (miRNAs) that regulate LIG1 and LIG3 and established a valuable miRNA–mRNA regulation network for BC. We found that the expression of LIG1 and LIG3 was upregulated in BC and predicted high relapse-free survival (RFS) in BC patients. Functional annotation analysis was performed to reveal the role of LIG1 and LIG3 in BC. In addition, hsa-miR-22-3p was identified to be potentially involved in the regulation of LIG3. We suggest that LIG1 and LIG3 are novel valuable prognostic biomarkers for BC and has-miRNA-22-3p may be a potential therapeutic target for BC.

Highlights

DNA ligase (LIG) plays a key role in connecting the 3′-OH end of a DNA strand to the 5′-P end of another DNA strand, resulting in the formation of a phosphodiester bond
The expression level of LIG1 was significantly higher in TCGA (p value = 0.002, fold change = 2.205) and Gluck breast (p value = 0.015, fold change = 2.349) [20] datasets compared with normal samples (Figure 1b)
In order to identify whether LIGs participated in the occurrence and progression of breast cancer (BC), the Oncomine database was used to analyze the expression of LIGs in BC and normal tissue samples

Summary

Introduction

Abstract: DNA ligase (LIG) plays a key role in connecting the 3′-OH end of a DNA strand to the 5′-P end of another DNA strand, resulting in the formation of a phosphodiester bond. It has been reported that LIGs (including LIG1, LIG3 and LIG4) play important roles in the occurrence and progression of many cancers. The role of LIGs in breast cancer (BC) is still unclear. We aim to reveal the expression level, function, and prognostic value of LIGs in BC. Bioinformatic tools were used to study the expression level, potential function and prognostic value of LIG1 and LIG3 in BC patients. We found that the expression of LIG1 and LIG3 was upregulated in BC and predicted high relapsefree survival (RFS) in BC patients. Functional annotation analysis was performed to reveal the role of LIG1 and LIG3 in BC. We suggest that LIG1 and LIG3 are novel valuable prognostic biomarkers for BC and has-miRNA-22-3p may be a potential therapeutic target for BC

Objectives

Results

Conclusion