Identification of LecRLK gene family in Cerasus humilis through genomic-transcriptomic data mining and expression analyses.

Hongyan Han,Zewen Wang,Pengfei Wang,Junjie Du,Xiaopeng Mu,Yu Gary Gao,Hongbo Fu,Debasis Chakrabarty

doi:10.1371/journal.pone.0254535

Hongyan Han, Zewen Wang + Show 6 more

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https://doi.org/10.1371/journal.pone.0254535

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Abstract

Lectin receptor-like protein kinases (LecRLKs) have been shown to be involved in plants’ responses to various biotic and abiotic stresse factors. Cerasus humilis is an important fruit species widely planted for soil and water conservation in northern China due to its strong tolerance to drought and salinity stresses. In this study, a total of 170 LecRLK family genes (125 G-types, 43 L-types and 2 C-types) were identified in the newly released whole-genome sequences of C. humilis. Furthermore, nine representative LecRLK genes in young plants of C. humilis under varying drought and salinity stresses were selected for qRT-PCR analysis. Our systematic comparative analyses revealed the active participation of these nine LecRLK genes in the salt and drought stress responses of C. humilis. The results from our study have provided a solid foundation for future functional verification of these LecRLK family genes and will likely help facilitate the more rapid and effective development of new stress resistant Cerasus humilis cultivars.

Highlights

During the whole life cycle of plants, both biotic and abiotic factors have significant influence on the growth, development and even survival of higher plants during their life cycle
The results from our study have provided a solid foundation for future functional verification of these Lectin receptor-like protein kinases (LecRLKs) family genes and will likely help facilitate the more rapid and effective development of new stress resistant Cerasus humilis cultivars
Two methods were used to generate the maximum number of LecRLK gene family members: Firstly, LecRLK genes in Prunus persica and Arabidopsis thaliana were used for local BLASTP in Genome of C. humilis

Summary

Introduction

During the whole life cycle of plants, both biotic and abiotic factors have significant influence on the growth, development and even survival of higher plants during their life cycle. Membrane localized receptor-like kinases (RLKs), together with other signal receptors like phytochromes, act as the first site of signal perception, which allows the plants to communicate between cells and to respond to changes in their environment [1,2]. Lectin receptor-like protein kinase (LecRLK) family is a group of RLKs that have been shown to play several vital roles in plant pathogenic defense, symbiotic association, insect feeding response, innate immunity and different abiotic stress responses, such as low temperature and high salinity [3,4,5,6,7,8,9,10,11,12,13,14].

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