Abstract
Background: Probiotics are non-pathogenic useful microorganisms having positive effects on the host health. The aim of the present study was to discriminate Lactobacillus species extracted from traditional dairy products. Methods: This study was conducted on 26 specimens collected from traditional dairy products in Bukan. Lactobacillus species were separated and purified employing biochemical tests. Then, the intra/inter-species diversity was investigated using RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) technique. Results: Polymorphism information content (PIC) value varied between 15.9% and 34.4% with a maximum value of 34.4% associated with primer 1254. The mean of and Marker index (MI) for the 6 primers was 4.52, in which the maximum and minimum values belonged to the primers 1254 and OPA-02, respectively. The isolates were categorized into 4 main clusters according to Jaccard similarity coefficient using UPGMA (unweighted pair group method with arithmetic means) clustering method. Principle coordinates analysis (PCoA) demonstrated that the first and second components explained 30.59% and 22.48% of variances, i.e. 53.07% of variances in total. The results of RAPD marker indicated that the intra-species diversity was greater than inter-species diversity. The intra-group variance explained 94% of the all variance, while inter-group variance explained only 6% of the all variance. Moreover, the results of analysis of molecular variance (AMOVA) indicated that the highest level of discrimination occurred at the 16 groups cut-off point with a similar coefficient of 0.56. Conclusions: From the results of the present study, it can be concluded that traditional dairy products are enriched sources of probiotic bacteria which can ensure the health of general population and enhance their immunoe systems. Moreover, RAPD-PCR is an appropriate method for detection and calssification of lactobassiluses.
Highlights
Probiotics are non-pathogenic useful microorganisms having positive effects on the host health
The Comparison of DNA Segments Produced by random amplified polymorphic DNA (RAPD) Reagent The 6 primers used in this study produced appropriate multi-shape bands encoded within a range from 200 bp to 3000 bp
A total number of 111 amplified sites were observed out of which 110 ones were of the polymorphism type
Summary
Probiotics are non-pathogenic useful microorganisms having positive effects on the host health. The aim of the present study was to discriminate Lactobacillus species extracted from traditional dairy products. The intra/inter-species diversity was investigated using RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) technique. The results of RAPD marker indicated that the intra-species diversity was greater than inter-species diversity. The results of analysis of molecular variance (AMOVA) indicated that the highest level of discrimination occurred at the 16 groups cut-off point with a similar coefficient of 0.56. Conclusions: From the results of the present study, it can be concluded that traditional dairy products are enriched sources of probiotic bacteria which can ensure the health of general population and enhance their immunoe systems. The most common bacteria used as probiotics are driven from lactic acid bacteria family and can normally be found in dairy products such as yoghurt, cheese, etc. The genus Lactobacillus bacterium, as a lactic acid bacterium, has attracted more attention than do other species of the family [5]
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