Identification of lactic acid bacteria from etawa goat milk kopelma Darussalam Village, Banda Aceh

Abstract

Identification of Lactic Acid Bacteria (LAB) has been carried out based on the catalase test and Gram staining of Etawa pure Goat Milk samples that obtained from Kopelma Village, Darussalam District, Banda Aceh City, Aceh Province. De Man, Rogosa, Sharpe Agar (MRSA) media was used for LAB isolation. There were 10 isolates of goat milk bacteria that used for microscopic characteristics, morphological observations, catalase test and Gram staining. Based on the microscopic test, it was explained that all colonies had similar characteristics, that having a round shape, convex surface shape, 1-2 mm diameter and cream color. In the catalase test, the results were negative, because all isolates did not produce gas bubbles after dripped with H2O2 solution. The morphological results showed that all isolates were Gram positive, marked by purple cells and bacilli cells. Based on the Gram staining and the shape of the bacillus cells, it were suspected that milk isolates are candidates for the genus Lactobacillus. Microscopic diagnosis is only a provisional presumption. Therefore, it needs a conclusive diagnosis, such as biochemical properties which are important factors that must be carried out for the next stage.