IDENTIFICATION OF JAVAN LANGUR (Trachypithecus auratus) IN JAVAN LANGUR CENTER (JLC) COBAN TALUN, BATU BASED ON Cytochrome-b SEQUENCE

Abstract

<p>Javan Langur (Trachypithecus auratus) is an endemic langur in Indonesia which classified as vulnerable primate. Some researches divided Javan Langur species into some subspecies, yet was not described properly. Cytochrome-b sequence from mitochondrial DNA is able to determine samples between and intra-species as well. This study aimed to identify the Javan Langur (Trachypithecus auratus) samples named Andin (@&) and Bobby (B&), both are rehabilitated in Javan Langur Center (JLC). The amplification of Cytochrome-b gene from whole blood DNA with PCR technique using forward primer L151625’­CTTCCATGAGGACAAATATC-3’ and reverse primer Rmuc15’-GTGGAGTATAGGTATGATTGC-3’. The phylogenetic tree reconstruction based on MEGA 5 using maximum likelihood (ML) method and pairwise distance analysis with Kimura-2 parameter model resulted that Andin and Bobby are in the same group with <br />T. a. auratus. Andin is in the same clade with T. a. auratus haplotype aaF, while Bobby in the same clade T. <br />a. auratus and T. a. auratus haplotype aaC. We concluded that both of them belong to T. a. auratus subspecies.</p><p><br /><strong>Keywords</strong>: Javan Langur (Trachypithecus auratus), Javan Langur Center (JLC), Cytochrome-b</p>