Identification of human TFH-specific miRNAs and investigation of their role in TFH differentiation and function

Abstract

Event Abstract Back to Event Identification of human TFH-specific miRNAs and investigation of their role in TFH differentiation and function Anna Ripamonti1, Elena Provasi1, Grazisa Rossetti1, Serena Curti1, Valeria Ranzani1, Valeria Parente1, Federica Facciotti1, Monica Moro1, Sergio Abrignani1 and Massimiliano Pagani1* 1 Istituto Nazionale Genetica Molecolare INGM, Italy Follicular helper T cells (Tfh) are a separate CD4+ T helper subset specialized in provision of help to B cells. They develop independently from other T cell subsets and are critical for humoral immunity, including the generation of long-lived and high affinity plasma cells and memory B cells. Alteration of Tfh-specific genes leads to defects in germinal centers (GCs) formation, with consequent autoimmune or immunodeficiency disorders. The molecular mechanisms underlying Tfh cells differentiation are poorly understood. MicroRNAs (miRNAs) are highly conserved non-coding single-stranded small RNA molecules that control gene expression post-transcriptionally by binding the 3′ untranslated region of target mRNA. To investigate the role of miRNAs in Tfh cells biology we performed RT-qPCR and deep sequencing analysis on Tfh and Naive CD4+ T cells sorted from human adenoids. We identified Tfh-specific miRNAs and in vitro validated their predicted targets. We found that Tfh-specific miRNAs regulate transcripts specifically expressed in Tfh cell subset and known to be essential for their function. We are performing gain or loss of function experiments using lentiviral vectors aimed at modulating miRNA expression in human Tfh cells. Through these experiments we will assess how these Tfh specific-miRNAs influence the ability of Tfh cells to help B cells, and elucidate their role in Tfh biology. Keywords: T follicular helper cells, MicroRNAs, germinal centers, B cell help, humoral immunity Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Ripamonti A, Provasi E, Rossetti G, Curti S, Ranzani V, Parente V, Facciotti F, Moro M, Abrignani S and Pagani M (2013). Identification of human TFH-specific miRNAs and investigation of their role in TFH differentiation and function. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01186 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 22 Jul 2013; Published Online: 22 Aug 2013. * Correspondence: Mr. Massimiliano Pagani, Istituto Nazionale Genetica Molecolare INGM, Milan, Italy, pagani@ingm.org Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Anna Ripamonti Elena Provasi Grazisa Rossetti Serena Curti Valeria Ranzani Valeria Parente Federica Facciotti Monica Moro Sergio Abrignani Massimiliano Pagani Google Anna Ripamonti Elena Provasi Grazisa Rossetti Serena Curti Valeria Ranzani Valeria Parente Federica Facciotti Monica Moro Sergio Abrignani Massimiliano Pagani Google Scholar Anna Ripamonti Elena Provasi Grazisa Rossetti Serena Curti Valeria Ranzani Valeria Parente Federica Facciotti Monica Moro Sergio Abrignani Massimiliano Pagani PubMed Anna Ripamonti Elena Provasi Grazisa Rossetti Serena Curti Valeria Ranzani Valeria Parente Federica Facciotti Monica Moro Sergio Abrignani Massimiliano Pagani Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.