Identification of Hiochi Bacteria by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoretic Patterns of Whole Cell Proteins.

Abstract

The whole cellular proteins of various hiochi bacteria were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). On the basis of the electrophoretic patterns, the hiochi bacteria were generally divided into four distinguishable groups, agreeing with the conventional groupings based on physiological and biochemical characters; homo- and hetero-fermentative true hiochi bacilli and homo- and hetero-fermentative hiochi lactobacilli. The SDS-PAGE patterns of the other lactobacilli were clearly different from that of the hiochi bacteria. The SDS-PAGE patterns of SDS-extracted protein from hiochi bacteria can be used as a simple and a useful tool for identification of the microorganisms causing putrefaction of sake.