Abstract
<p>Genes which were associated with flowering time to indicate the early maturity is known as heading date (<em>Hd</em>). Heading date six (<em>Hd6)</em> gene<em> </em>was identified from rice mutant varieties were Atomita 2, Atomita 3, Atomita 4, Bestari, Cilosari, Diah Suci, Sidenuk, Kahayan, Mayang, Meraoke, Mira-1, Pandan Putri, Superwin, Suluttan Unsrat 1, Suluttan Unsrat 2, Winongo, Woyla, Yuwono, while the rice var. Nipponbare was used as a positive control. All of rice mutant varieties derived from mutation induction by the dose of 0.2 kGy. The aim of this experiment was to find out the data base of mutant varieties which could be used as parent material with earlier maturity trait genetically. To obtain the DNA of plants, young leaves of each variety were extracted by liquid nitrogen, and then lysis and extracted by Kit Plant Genomic DNA. The amplification of DNA with 7 primers of <em>Hd6</em> conducted of 40 cycles by PCR and were continues to separated by 1 % agarose. The results were shown that the rice Mira-1 and Bestari varieties obtained from mutation of Cisantana highly different from one to another on 7 primers of <em>Hd6</em> used. Mayang variety from mutation of cross breeding between Cilosari and IR64, Pandan putri from Pandan wangi and Woyla from mutation of cross breeding from Atomita 2 and IR64 were highly different with those of their parents. Identification of <em>Hd6</em> gene on Sidenuk variety was shown the same bands pattern with Nipponbare as control positive toward all primers used, this variety would be better for earlier maturity parent material compared to others. The information could be useful for breeding programs aiming to develop early maturing widely adaptive and high yielding rice cultivars.</p>
Highlights
Rice is staple food for Indonesian people
One of treat to increase the rice production was Harvesting Index Program (IP 400) which was released by the government several years ago
Genes which were associated with flowering time to indicate the early maturity is known as heading date (Hd) (Takahashi and Shimamoto, 2011; Higashi and Izawa, 2011)
Summary
Rice is staple food for Indonesian people. The population of Indonesia is 254 million people and needs 35.56 million ton rice, supply from national rice production lower than rice consumption. Identification of heading date six genes in rice mutant varieties have never been studied, from this study, it would be differentiate between parents and their mutants and different among tested rice varieties genetically, and could be used for earlier mature parent plant genetically This analysis can lead to the development rice mutant varieties for generation sequencing from within the varieties. It would be understood the mutation occur in exon of rice mutant varieties, and allow us to evaluate and analyze variations for their similarity, and the differences reveal potential functional information The objective of this experiment to find out the data base of heading date gene of mutant varieties will answer these challenges and making it useful for development of early maturity seed
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