Abstract
Background and Aims: Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder. However, the underlying mechanism of IBS is not fully understood. The aim of this study was to investigate potential mechanism and novel biomarkers of IBS through evaluation of the metabolomic and microbiologic profile.Methods: Fecal samples were collected from 15 irritable bowel syndrome patients and 15 healthy controls. By using gas chromatography coupled to time-of-flight mass spectrometry (GC-TOFMS) and 16S rDNA amplicon sequencing, fecal metabolites and microbiota of healthy controls and the IBS patients were measured.Results: IBS patients had a significantly differential metabolite profile as compared to healthy controls, and 4 clusters with 31 metabolites, including a group of amino acids and fatty acids, were significantly up-regulated as compared to the healthy controls. In addition, 19 microbes were significantly up-regulated, and 12 microbes were down-regulated in the IBS group, when compared with the healthy controls. Some clusters of fecal metabolites or microorganisms were significantly correlated with the severity of IBS symptoms, such as the frequency of abdominal pain/discomfort and the number of bowel movements. Correlation of the metabolite levels with abundances of microbial genera showed some statistically significant metabolite-microbe associations. Four differentially abundant amino acids clustered together were positively correlated with some microbes, including Lachnospira, Clostridium, and so on.Conclusion: The finding of this study puts a global perspective on metabolomics and microbiota profiling in IBS patients and provides a theoretical basis for future research on pathophysiology of IBS.
Highlights
Irritable bowel syndrome (IBS) is one of the most common functional bowel disorders, affecting 10–15% of the general population worldwide (Lovell and Ford, 2012a,b)
IBS can be clinically subtyped into IBS with constipation (IBS-C), IBS with diarrhea (IBS-D) and mixed IBS (IBS-M) (Guilera et al, 2005; Oswiecimska et al, 2017)
Thirty adults were enrolled in this study, with 15 IBS patients meeting the Rome III criteria and another 15 healthy controls (HC)
Summary
Irritable bowel syndrome (IBS) is one of the most common functional bowel disorders, affecting 10–15% of the general population worldwide (Lovell and Ford, 2012a,b). It is characterized by chronic and recurrent abdominal pain/discomfort and disordered bowel habits in the absence of other organic gastrointestinal disease (Mayer, 2008; Ford et al, 2017). Previous studies of IBS mainly focused on the altered gastrointestinal motility, increased gut sensitivity, brain-gut interaction, and psychosocial distress (Kellow and Phillips, 1987; Rogers et al, 1989; Mayer and Tillisch, 2011). Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder. The aim of this study was to investigate potential mechanism and novel biomarkers of IBS through evaluation of the metabolomic and microbiologic profile
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