Identification of GSX2 and AF10 as NUP98 partner genes in myeloid malignancies

G Soler,V Penard-Lacronique,I Radford,O A Bernard,E Delabesse,C Broccardo,S Kaltenbach,S P Romana,M-J Mozziconacci,S Dobbelstein

doi:10.1038/bcj.2013.20

Abstract

Identification of GSX2 and AF10 as NUP98 partner genes in myeloid malignancies

Highlights

Both ALL-1 fused gene from chromosome 10 (AF10) and GSX2 genes have been involved in human hematological malignancies
The transformation potential of the two Nucleoporin 98 gene (NUP98) fusions was investigated by transducing murine primary bone marrow (BM) hematopoietic progenitors, defined as lineage-negative cells, by murine stem cell virus (MSCV) retroviral vectors containing different versions of Flag-tagged NUP98–GSX2 and NUP98–AF10 sequences as reported.[10]
Cells were transduced with MSCV expressing the native NUP98–GSX2 and NUP98–AF10 fusions, fusions deleted for the conserved functional domains (NUP98–GSX2-#HD and NUP98–AF10-DOM-LZ) or with the empty MSCV

Summary

Introduction

Both AF10 and GSX2 genes have been involved in human hematological malignancies. Cytological analysis of the colonies showed that cells expressing NUP98–GSX2 exhibit a blast morphology, whereas empty MSCV-transduced progenitors formed monocytic and mast cell colonies (not shown).

Results

Conclusion