Identification of growth hormone receptor (GHR|AluI) gene polymorphism in grati-madura cattle and pamekasan-madura cattle population

Abstract

The growth traits were one of the important economic characteristics in beef cattle. One of the growth genes that was suspected as a gene candidate for marker assisted selection (MAS) in Madura cattle was the growth receptor gene (GHR). This study aim was to detect the level of GHR gene polymorphism in Grati-Madura cattle and Pamekasan-Madura cattle populations. A total 86 blood samples of Madura cattle have been collected from the experimental barn of Beef Cattle Research Station and 51 blood samples of Madura cattle from Community Farms in Waru Subdistrict, Pamekasan Madura Regency. Blood samples were isolated using a zymo extraction kit. Detection of GHR growth hormone gene diversity using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method with AluI restriction enzymes. Data analysis used Chi-Square test for genotype, allele frequencies and Hardy Weinberg Equilibrium (HWE). The analysis result showed that GHR genes was detected 3 genotypes namely AA, AG and GG, each with an allele frequency of 0.267, 0.733 and 0.559, 0.441, this shows that the population of Grati-Madura cattle and Pamekasan-Madura cattle were polymorphic with PIC values of 0.315 and 0.372 respectively and were in the moderate category. The observed genotype frequencies in Grati Madura population deviated from HWE, while in Pamekasan madura population was not deviated from HWE. The value of Ho, He PIC and Ne were 0.394, 0.392, 0.315, 1.886 and 0.498, 0.493, 0.372, 1.973 respectively. In conclusion, The GHR gene polymorphism in Grati-Madura cattle and Pamekasan-Madura cattle were polymorphic and very informative so that can be used as gene candidate for MAS.