Identification of glycoproteins in mucous cells of the gill epithelium of Colossoma macropomum after exposure to organophosphate

S.E Moron,M.G.T Gomes,P.R Matos,A.T Ramos

doi:10.1590/1678-4162-9101

Abstract

ABSTRACT The use of organophosphates has been recommended for fish, especially the trichlorfon to control parasites. Colossoma macropomum were exposed to trichlorfon during 96 hours and of total number of mucous cells decreased in the number of cells when compared to the control group. Glycoproteins acid, acid sulphated and neutral was identified in the gill epithelium. Neutra glycoprotein had a significant decrease between control and the sublethal concentration. Acid glycoprotein didn’t have any significant difference between the groups exposed to the trichlorfon, compared to the control group. Sulfated acidic glycoprotein in the group exposed to the trichlorfon was noticed a reduction in number of mucosal cells acidic sulphated. The differences between density cell and production glycoprotein was a response of these cells after exposure to xenobiotic. The reduction of neutral, acid and sulphated acid glycoprotein in the MC of the gill epithelium Colossoma macropomum may affect gills epithelial surface protection by reducing the formation of an unstirred layer and enhance the ion loss.

Highlights

In the fish, the mucous cells are unique in their histological characteristics as size, numbers and studies have been carried out on the fish skin’s mucous cells (Diaz et al, 2005).The mucus, secreted by the mucous cells, on the gills surface has been considered an important protection against abrasive injuries by solid materials suspended in water, pathogenic bacteria, parasites (McCahon et al, 1987; Deszfuli et al, 2003), and pollutant (Ledy et al, 2003; Roberts and Powell, 2003)
The organophosphate insecticides are used in the control of odonata, an insect which is the main predator of fish (Mataqueiro, 2002) and used in the treatment and in the control of other predators (Carr and Chambers, 1996)
The fish (150.0±10g) were divided into two groups of 10 individuals each and each group: control group and test groupanimals treated with pesticide NEGUVON® 0,25mg

Summary

Introduction

The mucus, secreted by the mucous cells, on the gills surface has been considered an important protection against abrasive injuries by solid materials suspended in water, pathogenic bacteria, parasites (McCahon et al, 1987; Deszfuli et al, 2003), and pollutant (Ledy et al, 2003; Roberts and Powell, 2003). The organophosphate insecticides are used in the control of odonata, an insect which is the main predator of fish (Mataqueiro, 2002) and used in the treatment and in the control of other predators (Carr and Chambers, 1996). In fish farming they tend to eliminate and control ectoparasites (Rodrigues et al, 1997). The effects caused by the exposure to organophosphates have been broadly studied (Pope, 1999; Fulton and Key, 2001; Fanta et al, 2003; Roex et al 2003; Aguiar et al, 2004; Mohammad et al, 2004; Chandrasekara and Pathiratne, 2005; Thomaz et al, 2009)

Objectives

Methods

Results