Abstract
Glutathione peroxidases (GPXs) are important enzymes in the glutathione-ascorbate cycle for catalyzing the reduction of H2O2 or organic hydroperoxides to water. GPXs play an essential role in plant growth and development by participating in photosynthesis, respiration, and stress tolerance. Rhodiola crenulata is a popular traditional Chinese medicinal plant which displays an extreme energy of tolerance to harsh alpine climate. The GPXs gene family might provide R. crenulata for extensively tolerance to environment stimulus. In this study, five GPX genes were isolated from R. crenulata. The protein amino acid sequences were analyzed by bioinformation softwares with the results that RcGPXs gene sequences contained three conserve cysteine residues, and the subcellular location predication were in the chloroplast, endoplasmic reticulum, or cytoplasm. Five RcGPXs members presented spatial and temporal specific expression with higher levels in young and green organs. And the expression patterns of RcGPXs in response to stresses or plant hormones were investigated by quantitative real-time PCR. In addition, the putative interaction proteins of RcGPXs were obtained by yeast two-hybrid with the results that RcGPXs could physically interact with specific proteins of multiple pathways like transcription factor, calmodulin, thioredoxin, and abscisic acid signal pathway. These results showed the regulation mechanism of RcGPXs were complicated and they were necessary for R. crenulata to adapt to the treacherous weather in highland.
Highlights
Rhodiola crenulata belongs to the Rhodiola (Crassulaceae) species which is a popular medicinal plant and has been used in traditional Chinese medicine for thousands of years [1]
Primers were designed according to the ORF and sequences were validated by specific primer amplification products with Sanger sequencing
There were five unigenes annotated to Glutathione peroxidases (GPXs), compared with eight members for Arabidopsis thaliana GPXs
Summary
Rhodiola crenulata belongs to the Rhodiola (Crassulaceae) species which is a popular medicinal plant and has been used in traditional Chinese medicine for thousands of years [1]. The roots and rhizomes of Rhodiola crenulata contained several active ingredients like salidrosid, tyrosol, and their derivates which have been examined for adaptogenic and stress protection [2,3,4]. Rhodiola crenulata is widespread in Qinghai-Tibet Plateau (QTP) and its adjacent area with high elevations of 3500–5000 m [5]. The R. crenulata growing environment is alpine climate with characteristics of cold, drought, strong ultraviolet rays, and hypoxia [7]. R. crenulata is a perennial herb which could adapt to multiple stresses [8]. R. crenulata employed a different carbon dioxide fixation pathway by crassulacean acid metabolism which could maintain the photosynthesis in extreme environments. Little is known about the adaptive mechanism of genetics until now
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
