Abstract
BackgroundTipburn, also known as leaf tip necrosis, is a severe issue in Chinese cabbage production. One known cause is that plants are unable to provide adequate Ca2+ to rapidly expanding leaves. Bacterial infection is also a contributing factor. Different cultivars have varying degrees of tolerance to tipburn. Two inbred lines of Chinese cabbage were employed as resources in this research.ResultsWe determined that the inbred line ‘J39290’ was the tipburn resistant material and the inbred line ‘J95822’ was the tipburn sensitive material based on the severity of tipburn, and the integrity of cell membrane structure. Ca2+ concentration measurements revealed no significant difference in Ca2+ concentration between the two materials inner leaves. Transcriptome sequencing technology was also used to find the differentially expressed genes (DEGs) of ‘J95822’ and ‘J39290’, and there was no significant difference in the previously reported Ca2+ uptake and transport related genes in the two materials. However, it is evident through DEG screening and classification that 23 genes are highly linked to plant-pathogen interactions, and they encode three different types of proteins: CaM/CML, Rboh, and CDPK. These 23 genes mainly function through Ca2+-CaM/CML-CDPK signal pathway based on KEGG pathway analysis, protein interaction prediction, and quantitative real-time PCR (qRT-PCR) of key genes.ConclusionsBy analyzing the Ca2+ concentration in the above two materials, the transcription of previously reported genes related to Ca2+ uptake and transport, the functional annotation and KEGG pathway of DEGs, it was found that Ca2+ deficiency was not the main cause of tipburn in ‘J95822’, but was probably caused by bacterial infection. This study lays a theoretical foundation for exploring the molecular mechanism of resistance to tipburn in Chinese cabbage, and has important guiding significance for genetics and breeding.
Highlights
Tipburn, known as leaf tip necrosis, is a severe issue in Chinese cabbage production
Phenotypic analysis of two Chinese cabbage inbred lines—‘J95822’ and ‘J39290’ We studied two inbred line i.e.,‘J95822’ and ‘J39290’, to see how Chinese cabbage responded to tipburn
Incidence rate of tipburn in two Chinese cabbage inbred lines—‘J95822’ and ‘J39290’ We evaluated the overall tipburn incidence of Chinese cabbage varieties ‘J95822’ and ‘J39290’ during the rosette and heading stages, respectively
Summary
Known as leaf tip necrosis, is a severe issue in Chinese cabbage production. In the winter and spring, it is the most popular vegetable on people’s tables in North and Northeast China, and it has a large planting area. Yuan et al BMC Plant Biology (2021) 21:567. People prefer Chinese cabbage because it has a wide range of adaptation, can be widely planted, has rich nutrition, variety diversity, high yield and storage tolerance, and is high in carotenoids, protein, crude fibre, and other nutrients. The typical plant tipburn incidence rate of Chinese cabbage is 10-20%, and in severe circumstances, it can reach as high as 80% [3]. Farmers have suffered significant economic losses as a result of Chinese cabbage tipburn, which is getting increasingly severe. Tipburn starts in the rosette stage and becomes more serious as it progresses to the heading stage [3, 4]
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