Abstract
White birch (Betula platyphylla Suk.) is a monoecious tree species with unisexual flowers. In this study, we used a spontaneous mutant genotype that produced normal-like male (NLM) inflorescences and mutant male (MM) inflorescences at different locations within the tree to investigate the genes necessary for pollen development. A cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis was used to identify genes differentially expressed between the two types of inflorescences. Of approximately 5000 transcript-derived fragments (TDFs) obtained, 323 were significantly differentially expressed, of which 141 were successfully sequenced. BLAST analyses revealed 51.8% of the sequenced TDFs showed significant homology with proteins of known or predicted functions, 10.6% showed significant homology with putative proteins without any known or predicted function, and the remaining 37.6% had no hits in the NCBI database. Further, in a functional categorization based on the BLAST analyses, the protein fate, metabolism, energy categories had in order the highest percentages of the proteins. A Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the known TDFs were mainly involved in metabolic (28.4%), signal transduction (23.5%) and folding, sorting and degradation (13.6%) pathways. Ten genes from the NLM and MM development stages in the mutant were analyzed by quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR). The information generated in this study can provide some useful clues to help understand male sterility in B. platyphylla.
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