IDENTIFICATION OF GENE REGULATION PATTERNS BY INTERACTING EFFECTS OF CORTICOSTERONE AND THYROID HORMONE IN XENOPUS TROPICALIS TADPOLES.

Abstract

Event Abstract Back to Event IDENTIFICATION OF GENE REGULATION PATTERNS BY INTERACTING EFFECTS OF CORTICOSTERONE AND THYROID HORMONE IN XENOPUS TROPICALIS TADPOLES. Saurabh S. Kulkarni1* and Daniel R. Buchholz1 1 University of Cincinnati, Biological Sciences, United States Multiple hormones control the timing of amphibian metamorphosis. The main player is thyroid hormone (TH), which interacts with the end product of the stress axis corticosterone (CORT) to adjust the rate of development. However, the molecular mechanisms translating hormonal information into a developmental change are complex and not well understood. Therefore, to elucidate mechanisms of TH and CORT hormonal cross talk, we have identified patterns of gene regulation induced by CORT and/or TH in tadpole tails of X. tropicalis. We treated NF stage 54 tadpoles with four treatments: 1) CORT (100nm), 2) TH (50nM), 3) CORT+TH (100+50nm), and 4) vehicle for 18 hours followed by Affymetrix microarray analysis. The expression of a total of 3657 genes were significantly altered by ≥1.5 fold in response to either or both the hormones. Using Venn diagrams and cluster analysis, 19 distinct patterns of gene regulation were identified, based on up- or down-regulation by TH and/or CORT. We performed PCR analysis on 12 genes (2 genes from 6 patterns) to confirm results from the microarray. As expected, some genes were affected by only one of the hormones. We also found patterns of additive or subtractive synergy and antagonism. Surprisingly, the most of regulated genes, 2358 genes (64.5%), required both hormones for induction. These data imply that both hormones are important to achieve full induction of the gene regulation cascade underlying tail resorption and underscore the importance of hormonal cross talk when examining the molecular basis amphibian metamorphosis. Keywords: Amphibians, Corticosterone, metamorphosis, Microarray, Synergy, Tadpoles, Thyroid hormone, Xenopus Conference: ISAREN 2011: 7th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology, Ann Arbor, United States, 11 Jul - 13 Jul, 2011. Presentation Type: Poster Topic: Hormones and development Citation: Kulkarni SS and Buchholz DR (2011). IDENTIFICATION OF GENE REGULATION PATTERNS BY INTERACTING EFFECTS OF CORTICOSTERONE AND THYROID HORMONE IN XENOPUS TROPICALIS TADPOLES.. Front. Endocrinol. Conference Abstract: ISAREN 2011: 7th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology. doi: 10.3389/conf.fendo.2011.03.00024 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 22 Jul 2011; Published Online: 09 Aug 2011. * Correspondence: Mr. Saurabh S Kulkarni, University of Cincinnati, Biological Sciences, Cincinnati, United States, kulkarsb@mail.uc.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Saurabh S Kulkarni Daniel R Buchholz Google Saurabh S Kulkarni Daniel R Buchholz Google Scholar Saurabh S Kulkarni Daniel R Buchholz PubMed Saurabh S Kulkarni Daniel R Buchholz Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.