Identification of Foot and Mouth Disease (FMD) Virus From Recently Outbreak Crossbred Cattle In Rajbari District of Bangladesh

E Islam,Mh Rahman,M Giasuddin,Mz Hassan,Mr Akter,Mas Khan,A Chakrabartty,Mr Islam

doi:10.3329/sja.v19i1.54790

Abstract

Foot and mouth disease (FMD) is a devastating viral disease and endemic in nature in Bangladesh that causes huge economical losses. The present research work was aimed to determine the prevalence of FMD outbreaks and molecular detection of FMDV serotypes by uRT-PCR and gsRT-PCR test, respectively from crossbred cattle in the Rajbari district of Bangladesh during the period from January to June 2018. A total of 16 tongue epithelial samples were collected from clinically FMD suspected 2 to 3 years old crossbred cattle. 14 samples were positive by uRT-PCR. The detection rate of FMDV by uRT-PCR was 87.50%. Then uRT-PCR positive samples were serotype by gsRT-PCR. Serotype based prevalence of FMDV was 42.86%, 100%, 21.43% and 21.43% in O serotype, Asia-1 serotype, A serotype and mixed infection with Asia-1 and A, respectively. Considering the age, the prevalence of confirmed FMD outbreak was 42.86%, 35.71% and 21.43% at the age of 2, 2.5 and 3 years, respectively. Serotype A, O and Asia-1 is circulated in Rajbari district and required trivalent vaccine for prevention and control of FMD in that area. SAARC J. Agric., 19(1): 201-210 (2021)

Highlights

Foot and mouth disease (FMD) is, one of the world's most important contagious viral diseases, responsible for large-scale epidemics, huge global losses of livestock production and trade (Knowles and Sameul, 2003; Kitching et al, 2005; Samuel and Accepted: 30.06.2021Knowles, 2001; OIE, 2019)
The present research has been designed to determine the prevalence of FMD virus from a recent outbreak of crossbreed cattle and characterizing the serotypes of FMD virus circulating in naturally infected crossbred cattle by uRT-PCR and gs Reverse Transcription Polymerase Chain Reaction (RT-PCR), respectively
All of the 16 field samples of tongue epithelia were subjected to uRT-PCR for the detection of Foot and Mouth Disease Virus (FMDV) by yielding an amplicon size of 328 bp through 2% agarose gel electrophoresis (Figure-03)

Summary

Introduction

Foot and mouth disease (FMD) is, one of the world's most important contagious viral diseases, responsible for large-scale epidemics, huge global losses of livestock production and trade (Knowles and Sameul, 2003; Kitching et al, 2005; Samuel and Accepted: 30.06.2021Knowles, 2001; OIE, 2019). Foot and mouth disease (FMD) is, one of the world's most important contagious viral diseases, responsible for large-scale epidemics, huge global losses of livestock production and trade FMD is a threat to the livestock population throughout the world including Bangladesh due to its high contagiousness, a rapid multiplicity of the type and subtypes, wide species of animal’s susceptibility, short-lived immunity and the existence of persistent or carrier state (Bachrach, 1968; Saiz and Doming, 1996; Sevilla and Domingo, 1996). For control of FMD outbreaks or complete elimination of the virus, reliable, rapid and confirmatory diagnosis of the disease is important and outbreaks should be detected at an early stage and persistent infections should be recognized to prevent further transmittance. The present research has been designed to determine the prevalence of FMD virus from a recent outbreak of crossbreed cattle and characterizing the serotypes of FMD virus circulating in naturally infected crossbred cattle by uRT-PCR and gs RT-PCR, respectively

Methods

Results

Conclusion