Identification of Five Types Kampar River Fish With Genetic Approach

Abstract

Fish identification in the mainland waters of Riau Province is generally carried out based on morphological characteristics. To identify fish species accurately in a short time, the DNA Barcoding method with a molecular approach is used. Five types of fish were taken from Kampar River and coded as MJ313, PJ36, SJ35, BJ33, and GJ41. The genes of these five fish were amplified, and PCR products were sequenced and analyzed using bioinformatics software. The editing of the sequencing results and the determination of the nucleotide composition were analyzed using Mega5 software. DNA sequences were aligned using the ClustalW software. The sequences obtained are then compared with GeneBank data using Basic Local Alignment Search Tools (BLAST). The results showed that the species with the code MJ313 and GJ41 were Channa striata, PJ36 were Osteochilus hasseltii, BJ33 were Mystus sp. Based on the sample examination, the species coded SJ35 cannot be determined because the marker band is low so that it is not visible. The results of the analysis on species coded SJ35 indicate that there is no COI report for species coded SJ35 on GeneBank.