Identification of Farnesoid X Receptor as a Novel Nuclear Receptor Sensing for Gallstone Diseases

Abstract

Objective:-The bile acid-make active nuclear receptor farnesoid X receptor (FXR) acting an significant part in lipid then glucose metabolism, besides furthermore, it controls numerous lipid carriers complicated in stone disposition. We observed whether a useful single nucleotide polymorphism (SNP) in FXR (82 G>T) predisposed the gallstone development. Material and Method:- The polymorphism were confirmed by polymerase chain reaction monitored by PCR amplicons were commercially sequenced from both (forward and reverse) termini according to education manuals of the sequencing company (Macrogen Inc. Geumchen, Seoul, South Korea)., in two group match by age?20 : patient with gallstone (n=140) and stone-free control (n=140). Result :- For Sequencing of the 195 bp region within the NRIH4 (FXR) gene Within this locus, twenty samples were included in the present study that had shown to amplify the NR1H4 (or FXR) genetic sequences in the chromosome number 12. The latter gene is responsible for encoding on nuclear receptor subfamily 1 group H member 4 (NR1H4). The alignment results of the 195 bp samples revealed the presence of one SNP occurred in this position in sample no. 10 within the analyzed twenty samples in comparison with the referring reference DNA sequences The sequencing chromatogram of the observed substitution SNP, as well as its detailed annotations, were documented, and the chromatogram details of the observed SNP were shown according to their positions in the PCR amplicon, in which samples no. 10-20 had shown this (T82G )variation. Conclusion:- the education presented that the variation allele of the communal FXR 82G>T polymorphism was significantly associated with stone formation in Iraq patients. The association is possibly concluded the effect of the FXR 82G >T polymorphism on the expression of the efflux carriers for lipid trails in hepatic.