Identification of extracellular enzyme-producing bacteria (proteolytic, cellulolytic, and amylolytic) in the sediment of extensive ponds in Tanggulrejo, Gresik

Abstract

The process of aquaculture is an activity that potentially generates organic waste primarily derived from residual feed and the results of excretion (feces). Heterotrophic bacteria have the ability to produce extracellular enzymes (protease enzymes, amylases, and cellulases) that are necessary for the bioremediation of organic waste. The purpose of this study was to investigate the identifiable extracellular enzyme bacteria from extensive pond sediments in Tanggulrejo Village, Gresik. Twenty bacterial isolates were found from pond sediments that had the ability to produce extracellular enzymes and proteolytic, amylolytic and cellulolytic bacteria. The four isolates were identified as genera Bacillus thuringiensis, Bacillus lentus, Bacillus sphaericus and Corynbacterium pilosum. Bacillus thuringiensis bacteria have a hydrolysis zone of protein of 11 mm, a hydrolysis zone of amylum of 10 mm, and a hydrolysis zone of cellulose of 8 mm. Bacillus lentus bacteria have a hydrolysis zone of protein of 14 mm, a hydrolysis zone of amylum of 12 mm and a hydrolysis zone of cellulose of 7 mm.Bacillus sphaericus bacteria have a hydrolysis zone of protein 14 mm, a hydrolysis zone of amylum of 11 mm and a hydrolysis zone of cellulose of 6 mm. Corynebacterium pilosum bacteria have a hydrolysis zone of protein of 10 mm, a hydrolysis zone ofamylum of 14 mm and a hydrolysis zone of cellulose of 16 mm.